scTAPS-seq

DNA methylation

Assessing the properties of your samples on a single-cell level allows you to get an insight into the heterogeneity of your cell population. It helps entangle complex gene regulatory networks by identifying drivers of differential gene expression across cells, tissues or diseases. This includes cellular responses to stimuli or drugs, as these can vary substantially between cells. This plate-based epigenetic NGS method will provide researchers with a tool to assess DNA methylation of the genome or specific accessible regions of the chromatin.

From € 325.90/plate*

* excluding VAT and sequencing; on top of NlaIII/Karyo/ChIC processing costs
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Why

Cytosine methylations in DNA are important epigenetic factors. 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) are by far the most occurring variants. Methylation can induce or repress gene expression. Dysregulation of the methylome is known to play an important role in cancer (Wang & Lei, 2018).

How

scTAPS-seq is using similar protocols as scChIC-seq and scKaryo-seq. It enzymatically converts 5mC and 5hmC. Subsequently a PCR reaction converts these into thymines, creating a detectable C-to-T transition.

What

The gold standard for quantifying DNA methylation has been based on bisulfite conversion of genomic DNA. However, this harsh chemical treatment partly degrades DNA, hindering the detection of DNA methylation in single cells. Recently, a new bisulfite-free technology was developed based on pyridine borane reduction, which does not destroy the DNA (Liu et al., 2019). This method is called TAPS-seq for TET-assisted pyridine borane sequencing and was developed by the Song lab at Oxford University. The Van Oudenaarden lab recently started a collaboration with the Song lab and was able to successfully apply TAPS-seq to single cells (scTAPS-seq).

 

DNA methylation

Assess the epigenetic layer on top of the genome with scTAPS-seq by using CpG methylation information of the promotor region.

  • Evaluate the 5mC/5hmC methylation status of promotors within single cells
  • Bisulfite-free sequencing
  • Combine scTAPS with scNlaIII/scKaryo/scChIC-seq
Starts at

€325.90

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