Single-cell sequencing to unravel the underlying mechanisms of tumor development and drug resistance
Especially in cancer, acquired genetic alterations due to carcinogenic exposure and DNA replication infidelity play a key role in its initiation. As the tumor grows, these alterations lead to clones of the tumor with distinct genetic alterations (such as SNVs, CNVs and indels). This heterogeneity within the tumor is vital to tumor development, proliferation and metastatic potential. Furthermore, heterogeneity within the tumor plays a key role in therapy resistance. Investigating the (epi)genome of single cells is therefore important to understand heterogeneity within the tumor.
In recent years many novel sequencing technologies have been established in the research groups of Alexander van Oudenaarden and Geert Kops. These new technologies enable the quantification of the heterogeneity mentioned earlier. To make this technology widely available, Single-Cell Core was launched in September 2020. This facility offers a series of state-of-the-art single-cell genome sequencing technologies (scWGS, scChIC-seq and scKaryo-seq). Single-Cell Core, made possible by the Oncode Institute, will lower the barrier for researchers to perform these experiments, which require high technical expertise. This will enable researchers to access (epi)genomic detection in single cells.
Interested in other Oncode Institute technologies and facilities? Visit their website and find out more!
At this moment, Single-Cell Core focuses on (epi)genomic sequencing technologies. By the end of 2021, the facility aims to add more different technologies to its repertoire, allowing researchers to explore even more aspects of the (epi)genome and transcriptome.
Therefore we advise you to contact Single Cell Discoveries (SCD) for transcriptome-related research. SCD offers a variety of methods ranging from 3′ mRNA sequencing to full-length total RNA sequencing.
Interested in doing larger numbers of cells? SCD also offers 10X microfluidic solutions.
Together, Prof. dr. ir. Alexander Van Oudenaarden and Prof. dr. Geert Kops shaped and initiated Single-Cell Core. As experts in the field of single-cell sequencing, they provide the facility with state-of-the-art NGS technologies, allowing the facility to follow research trends and help researchers explore their samples in more depth.
Single-Cell Core is funded by the Oncode Institute and located within the KNAW Hubrecht Institute in Utrecht.
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