Assessing the properties of your samples on a single-cell level allows you to get an insight into the heterogeneity of your cell population. Looking at the whole genome of a single cell is vital to find copy number variations (CNVs) underlying different diseases, like cancer. With this plate-based single-cell NGS technology Single-Cell Core provides researchers with a tool to address this.
From € 487.22/plate*
* excluding VAT and sequencing
Maintenance of a diploid genome is essential to embryonic development and tissue homeostasis, but cancer genomes are marked by extensive chromosomal aberrations, including amplifications and deletions. These aberrant karyotypes show copy number alterations (CNAs) at the level of whole chromosomes (aneuploidy) or sub-chromosomal regions.
Nuclei in 384-well plates are digested with NlaIII, after which the genomic fragments (following end processing) are ligated to barcoded adapters containing a unique molecular identifier (UMI), cell-specific barcode, and T7 promoter allowing linear amplification by in vitro transcription (IVT).
To map cancer karyotypes at a single-cell level, scKaryo-seq, a whole genome sequencing approach, is used to assess CNAs at any preferred resolution. It probes karyotype heterogeneity in cancer and examines if and how this relates to tumor progression, therapy resistance and tumor relapse. It can be applied to sorted single cells, nuclei, organoids or tumor samples. Using scKaryo-seq at 2-5 Mb resolution, one can assess CNAs in thousands of cells from dozens of samples in one single-end sequencing run. scKaryo-seq can also be used to detect genomic mutations in single cells.
Identify copy number variations within a chromosome or at the whole chromosome level (aneuploidy) in the genome by profiling hundreds of single cells in parallel, and discover the underlying cause of cellular malfunctioning.