scNlaIII-seq

Whole genome sequencing

Assessing the properties of your samples on a single-cell level allows you to get an insight into the heterogeneity of your cell population. Looking at the whole genome of a single cell is vital to find single-nucleotide variations (SNVs) and copy number variations (CNVs) underlying different diseases, like cancer. With this plate-based single-cell NGS technology Single-Cell Core provides researchers with a tool to address this.

From € 324.57/plate*

* excluding VAT and sequencing
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Why

Tumor clones exhibit distinct genetic compositions of single nucleotide variants (SNVs) and insertions/deletions (indels). Intra-tumor heterogeneity plays a key role in cancer development. Tumor clones may have varying proliferative and metastatic potentials, for instance. This heterogeneity plays a key role in therapy resistance and the frequent lethal outcome of cancer.

How

Nuclei in 384-well plates are digested with NlaIII, after which the genomic fragments (following end processing) are ligated to barcoded adapters containing a unique molecular identifier (UMI), cell-specific barcode, and T7 promoter allowing linear amplification by in vitro transcription (IVT).

What

By enzymatically cutting the genomic DNA a well-defined sequencing start is obtained, which increases the overlap in coverage between individual cells. Other whole-genome single-cell sequencing methods that rely on random priming or random integration by transposases do not share this advantage. However, it is still very costly to sequence single-cell genomes for many cells. Single-Cell Core will therefore also provide a targeted-genome single-cell sequencing method by using bait oligos that can be used to enrich sequencing libraries for specific genomic regions. This helps us to enrich ~1000 genomic regions spanning about 1 Mb in total. This enrichment allows a 100-fold gain in sequencing depth and a corresponding 100-fold decrease in sequencing costs.

Whole genome sequencing

Identify genetic mutations by profiling hundreds of single cells in parallel and discover the underlying cause of cellular malfunctioning.

  • Detect SNVs within single cells
  • Low-cost WGS
  • Range of coverage for different applications
Starts at

€324.57

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